effects of culture media and growth regulators on micropropagation of gisela 6 rootstock

this study was conducted with the main prupose of investigation the effects of culture medium and the concentration of growth regulators on proliferation rate, rooting and the acclimatization of gisela 6 rootstock at khorasan razavi agriculture and natural resource research center during 2011-2012. in this experiment, the proliferation and rooting were performed in six kinds of culture medium (dkw, wpm and ms in two solid and liquid media) supplemented with plant growth regulators included tdz and bap in zero, one, two mg/l plus 0.01 mg/l iba in all treatments of the proliferation and iba with zero, one, two, three mg/l in rooting step. the results showed that the highest number and length of shoot were 6.48 shoot and 3.14 cm, respectively in solid wpm medium and two mg/l bap concentration and liquid ms medium with one mg/l concentration of tdz. the liquid ms medium in two mg/l of iba caused the highest percentage of rooting and root length, 93.90 percent in 11.76 cm, respectively. although applying the liquid media resulted in better acclimatization of plantlets, but the highest percent of plantlets survival was obtained in wpm liquid medium with two mg/l concentration of iba at about 91.80 percent.